CSU researchers uncover new information on replication of viruses
Inspired by previous works, the researchers screened a series of cyclic nitrogen-containing compounds and found that 1 H -1,2,3-triazole-4-carbaldehyde (TA4C) derivatives can be conjugated to the N-terminus in a single step with relatively high conversions, up to 92%.
"Simplifying protein modification is a valuable development for a variety of fields," corresponding author Akira Onoda explains. "Our approach results in highly efficient site-specific labeling under mild conditions, which is important when working with sensitive biological molecules. As long as the molecule to be added contains an amino group, a reaction can be carried out to create the TA4C group in one step, which is then reactive towards the protein N terminus."
The TA4C reagents are prepared in a single step from a functional molecule with an amino group via a reaction known as the Dimroth rearrangement. A variety of amine-containing molecules were successfully used, including polyethylene glycol, biotin, and fluorescein, demonstrating the wide range of possible functionalities.
We believe our approach will contribute as an immensely practical option to the protein modification toolbox and accelerate development in many areas which rely on protein conjugation. In addition, combining our approach with techniques that target other protein sites will allow multiple functions to be introduced, providing great flexibility. This will prove beneficial in a wide variety of fields including bioengineering, pharmaceuticals, and diagnostics." Takashi Hayashi, corresponding author
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