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[The effect of PM(2.5) on oncogene expression in HBE cells]. Abstract Source: Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2019 Aug 20 ;37(8):561-566. PMID: 31495106 Abstract Author(s): K Zheng, B Y Wang, X Y Xu, H Geng, D X Long Article Affiliation: K Zheng Abstract: To study the effect of particulate matter 2.5 (PM(2.5)) on oncogene expression in human bronchial epithelial (HBE) cells.HBE cells were selected as the study subjects, and PM(2.5) treatment group (10μg/ml and 50 μg/ml) , negative control group and positive control group (10 μmol/L Cr(6+)) were set. CCK8 assay was used to test the IC(50) value of PM(2.5). HBE cells were treated with PM(2.5) for 24 h at 10 μg/ml and 50 μg/ml, additionally, cells were treated with blank as negative control, 10 μmol/L Cr(6+) as a positive control for 24 h. After the treatment, mRNA expression of oncogenes including c-myc, c-fos, k-ras and p53 were detected by fluorescent quantitative RT-PCR, the protein expression of oncogenes were detected with western blot.The IC(50) value of PM(2.5) in HBE cells is 70.12μg/ml. The qRT-PCR data showed that compared with the control group, the expression level of c-myc gene increased by respectively 500.1%、780.7%、305.3% after exposure to 10、50 μg/ml PM(2.5) and positive control group; c-fos gene increased respectively 34.0%、76.7%、131.3% after exposure to10、50 μg/ml PM(2.5) and positive control group; k-ras gene increased respectively 50.3%、107.0%、49.7% after exposure to 10、50 μg/ml PM(2.5) and positive control group; p53 gene decreased by 28.3%、28.7%、59.7% after exposure to 10、50 μg/ml PM(2.5) and positive control group. The western blot results showed that compared with the control group, c-myc protein increased respectively 29.7%、77.3% after exposure to 50 μg/ml PM(2.5) and positive control group; c-fos protein increased respectively 200.3%、137.0% after exposure to 50 μg/ml PM(2.5) and positive control group; k-ras protein increased respectively 106.3%、130.3%、116.7% after exposure to 10、50 μg/ml PM(2.5) and positive control group; p53 protein decreased by 43.7%、53.3%、52.1% after exposure to 10、50 μg/ml PM(2.5) and positive control group.PM(2.5) could promote the expression of oncogenes in HBE cells, the carcinogenicity of haze might be related to promotion of oncogenes expression induced by PM(2.5). Aug 19, 2019 Study Type : Human In Vitro Additional Links Additional Keywords : Oncogenesis Promotion : CK(4) : AC(4) , Risk Factors : CK(3057) : AC(392) Print Options Some features are currently member only features. If you are already a member, please login . Otherwise, click here to become a member. Printer-friendly version