New label-free microscopic techniques provide better visualization of extracellular vesicles

New label-free microscopic techniques provide better visualization of extracellular vesicles

Universal 'One-Size-Fits-All' Cancer Treatment The technique used by the lab involves using ultrashort laser pulses which interact with the tissue samples. "There are two mechanisms involved in imaging," You said. "Some of the tissue components emit different kinds of fluorescence which comes in different colors. The other mechanism involves molecular structures that when aligned in a certain way, will give you an entirely different set of colors." The researchers have also begun to characterize the contents of the vesicles using this technique. "We have found that the vesicles which have higher concentrations of NADPH molecules are highly correlated with tumor aggressiveness, and are often located around vessels. By characterizing the vesicles, we have a good shot at detecting cancerous changes at an earlier stage," You said. A second paper, "Real-Time Intraoperative Diagnosis by Deep Neural Network Driven Multiphoton Virtual Histology," was published in Precision Oncology , and it looks at combining the label-free imaging with deep learning techniques. Currently, when surgeons remove tumors from patients, they analyze the tissue samples from the margins of the tumor to ensure that all the cancer tissue has been removed. Unfortunately, the pathology labs that analyze the samples can take up to a few days to complete their analysis. "We are trying to use the label-free technique to look at the tissue right away in the surgical room," You said. "After we get the images we use deep learning, which can be used to differentiate between cancerous and normal breast tissue." "We hope that this technique will be used for medical diagnosis and more clinical applications. The current techniques are useful but outdated and time-consuming. We think that there is a lot of new information that we can gain from this technology," Boppart said. "The system itself is somewhat expensive, but not unlike other high-end microscopes currently in use, and costs should come down in a few years as the laser technology matures," Boppart said. "The other inherent challenge for us is that if you want high resolution images, you need to focus on smaller areas. However, when we look at the smaller areas, we can still get an adequate picture of what is going on in the tissue." Source: Journal reference: You, S, et al. (2020) Label-free visualization and characterization of extracellular vesicles in breast cancer. PNAS. doi.org/10.1073/pnas.1909243116 .



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