Sperm microbiome revealed with RNA sequencing
Viral infections are a leading cause of illness and deaths. The new coronavirus, for example, has led to more than 24,000 confirmed cases globally, including more than 3,200 severe ones and nearly 500 deaths as of Feb. 5, according to a World Health Organization report.
Influenza A, a highly contagious virus that arises every year, is concerning due to the unpredictable effectiveness of its vaccine. Influenza A mutates rapidly, growing resistant to drugs and vaccines as it replicates.
The new study highlights a promising new tool for virologists to study the behavior of influenza A, as well as any other RNA viruses, in host cells and to identify the external conditions or cell properties affecting them. Until now, studying mutations in cells has required destroying them to extract their contents. The new tool enables analysis without killing cells, allowing researchers to get snapshots of viral replication as it occurs. Next steps include studying multiple segments of viral RNA and monitoring the influenza A virus in animals. Source:
Rutgers University Journal reference:
Dardir, K., et al. (2020) SERS Nanoprobe for Intracellular Monitoring of Viral Mutations. Journal of Physical Chemistry C . doi.org/10.1021/acs.jpcc.9b09253 .
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