An interdisciplinary team from Frankfurt and Jena has developed a kind of bait with which to fish protein complexes out of mixtures. Thanks to this "bait", the desired protein is available much faster for further examination in the electron microscope. The research team has christened this innovative layer of ultrathin molecular carbon the "smart nanosheet". With the help of this new development, diseases and their treatment with drugs can be better understood, for example.
With our process, new types of proteins can be isolated from mixtures and characterized within a week. To date, just the isolation of the proteins was often part of a doctorate lasting several years." Daniel Rhinow, Max Planck Institute of Biophysics in Frankfurt
Together with Andreas Terfort (Goethe University) and Andrey Turchanin (Friedrich Schiller University Jena), the idea evolved a few years ago of fishing the desired proteins directly out of mixtures by equipping a nanosheet with recognition sites onto which the target protein bonds. The researchers have now succeeded in making proteins directly available for examination using electron cryo-microscopy through a "smart nanosheet".
Electron cryo-microscopy is based on the shock-freezing of a sample at temperatures under -150 °C. In this process, the protein maintains its structure, no interfering fixing and coloring agents are needed, and the electrons can easily irradiate the frozen object. The result is high-resolution, three-dimensional images of the tiniest structures - for example of viruses and DNA, almost down to the scale of a hydrogen atom.
In preparation, the proteins are shock-frozen in an extremely thin layer of water on a minute metal grid. Previously, samples had to be cleaned in a complex procedure - often involving an extensive loss of material - prior to their examination in an electron microscope. The electron microscopy procedure is only successful if just one type of protein is bound in the water layer. Related Stories
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