Genome-Wide Methylation Analysis Identifies NOX4 and KDM5A as Key Regulators in Inhibiting Breast Cancer Cell Proliferation by Ginsenoside Rg3.

Genome-Wide Methylation Analysis Identifies NOX4 and KDM5A as Key Regulators in Inhibiting Breast Cancer Cell Proliferation by Ginsenoside Rg3.

April 03, 2019 0 Comments

Ginsenoside Rg3 is a key metabolite of ginseng and is known to inhibit cancer cell growth. However, the epigenetics of CpG methylation and its regulatory mechanism have yet to be determined. Genome-wide methylation analysis of MCF-7 breast cancer cells treated with Rg3 was performed to identify epigenetically regulated genes and pathways. The effect of Rg3 on apoptosis and cell proliferation was examined by a colony formation assay and a dye-based cell proliferation assay. The association betwee...

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20(s)-ginsenoside Rg3-loaded magnetic human serum albumin nanospheres applied to HeLa cervical cancer cells in vitro.

20(s)-ginsenoside Rg3-loaded magnetic human serum albumin nanospheres applied to HeLa cervical cancer cells in vitro.

April 03, 2019 0 Comments

20(s)-ginsenoside Rg3 is extracted from traditional Chinese medicine, red ginseng. However, due to its poor aqueous solubility and low oral bioavailability, the use of 20(s)-Rg3 is limited. This study aimed to explore a method of preparing nano-sized 20(s)-ginsenoside Rg3 particle named 20(s)-ginsenoside Rg3-loaded magnetic human serum albumin nanospheres (20(s)-Rg3/HSAMNP) to change dosage form to improve its aqueous solubility and bioavailability. 20(s)-Rg3/HSAMNP were prepared by the desolvat...

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Seroprevalence, cross antigenicity and circulation sphere of bat-borne hantaviruses revealed by serological and antigenic analyses

Seroprevalence, cross antigenicity and circulation sphere of bat-borne hantaviruses revealed by serological and antigenic analyses

April 03, 2019 0 Comments

http://orcid.org/0000-0002-5322-1435 http://orcid.org/0000-0002-2844-9673 http://orcid.org/0000-0001-7587-152X Lin Xu,  Jianmin Wu,  Qi Li,  Yamei Wei,  Zhizhou Tan,  Jianqiu Cai,  Huancheng Guo,  Ling’en Yang,  Xiaohong Huang,  Jing Chen Bats are newly identified reservoirs of hantaviruses (HVs) among which very divergent HVs have been ...

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Generating and Working with Drosophila Cell Cultures - Advanced Science News

Generating and Working with Drosophila Cell Cultures - Advanced Science News

April 03, 2019 0 Comments

The use of Drosophila cell cultures has positively impacted both fundamental and biomedical research. The most widely used cell lines—Schneider, Kc, the CNS and imaginal disc lines—continue to be the choice for many applications. To improve the state of Drosophila cell cultures, researchers from the Drosophila Genomics Resource Center, a NIH-funded repository for Drosophila DNA and cell line resources at the Indiana University Bloomington, review the current challenges and opportunities in this ...

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Synergy between probiotic Lactobacillus casei and milk to maintain barrier integrity of intestinal epithelial cells.

Synergy between probiotic Lactobacillus casei and milk to maintain barrier integrity of intestinal epithelial cells.

April 03, 2019 0 Comments

We hypothesized that Lactobacillus casei BL23 and milk work synergistically to prevent damage to epithelial barrier integrity induced by pro-inflammatory cytokines. To test this, barrier disruption was induced in polarized Caco-2 monolayers by sequential, basolateral treatment with IFN-γ and TNF-α. Apical application of either 25% v/v reconstituted skim milk (RSM) or Ultra High Temperature (UHT) milk (2% fat) prior to cytokine exposure reduced losses to transepithelial electrical resistance (TER...

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Mechanisms of synergistic neurotoxicity induced by two high risk pesticide residues - Chlorpyrifos and Carbofuran via oxidative stress.

Mechanisms of synergistic neurotoxicity induced by two high risk pesticide residues - Chlorpyrifos and Carbofuran via oxidative stress.

April 03, 2019 0 Comments

Multi-component pesticide residues, especially pesticide residues with synergistic toxicity, are a serious threat to food safety. With risk assessment, we found that Chlorpyrifos (CPF) and Carbofuran (CBF) are 2 pesticide residues with highest risk for Actinidia chinensis planch. The results showed CPF and CBF have a synergistic neurotoxicity on neural cell SK-N-SH. The toxicity was partly depending on oxidative stress (OS) and had effects on cell apoptosis and cell cycle arrest. Furthermore, th...

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Lymphocytes DNA damages and exposure to chlorpyrifos, deltamethrin, penconazole, copper oxicloride.

Lymphocytes DNA damages and exposure to chlorpyrifos, deltamethrin, penconazole, copper oxicloride.

April 03, 2019 0 Comments

PURPOSE:The evaluation of the association between different exposure to pesticides and different types of comet images among a group of 26 recruited subject. MATERIAL AND METHODS:We have recruited 26 subjects (farmers and not farmers). They were submitted to a blood drawing of 10 mL and a questionnaire was administered to investigate personal habits and work history. By means of the comet assay it was assessed the oxidative DNA damage of peripheral lymphocytes by pesticides in the exposed a...

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Vitamin C Prevents Hydrocortisone-Induced Injury in HMEC-1 through Promoting Bestrophin-3 Expression.

Vitamin C Prevents Hydrocortisone-Induced Injury in HMEC-1 through Promoting Bestrophin-3 Expression.

April 03, 2019 0 Comments

OBJECTIVE:To investigate the protective effects and underlying mechanisms of Vitamin C (VC) on hydrocortisone (HC)-induced cell injury in human microvascular endothelial cells (HMEC). METHODS:Cell viability was measured by CCK-8 assay and the expression of Best-3 was detected by Western blotting assay. The experiment was divided into normal control, HC injury group, VC treatment groups, HC + Best-3 siRNA group, HC + VC + Best-3 siRNA group, HC + pcDNA3.1 Best-3 group, and HC + VC + pcDNA3.1...

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Basic Cell Culture Protocols

Basic Cell Culture Protocols

April 03, 2019 0 Comments

Goal amount for this month: 30 USD, Received: 0 USD (0%) Download Basic Cell Culture Protocols inside Othersforums : Basic Cell Culture ProtocolsByCheryl D. Helgason (auth.), Cheryl D. Helgason, Cindy L. Miller (eds.) 2005 | 371 Pages ... Basic Cell Culture ProtocolsByCheryl D. Helgason (auth.), Cheryl D. Helgason, Cindy L. Miller (eds.) 2005 | 371 Pages | ISBN: 1588292843 | PDF | 29 MB In this fully revised edition of an established classic, expert researche...

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DNA Methylation and Cancer Therapy

DNA Methylation and Cancer Therapy

April 03, 2019 0 Comments

Goal amount for this month: 30 USD, Received: 0 USD (0%) Download DNA Methylation and Cancer Therapy inside Othersforums : DNA Methylation and Cancer TherapyByMoshe Szyf Ph.D. (auth.) 2005 | 239 Pages | ISBN: 030647848X | PDF | ... DNA Methylation and Cancer TherapyByMoshe Szyf Ph.D. (auth.) 2005 | 239 Pages | ISBN: 030647848X | PDF | 6 MB NA methylation has bewildered molecular biologists since Hotchkiss discovered it almost six decades ago (Hotchkiss RDJ. B...

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